Description, Instructions, and Tips for DB-Stat

Instructions for ProteinProspector Programs

Contents of this document:

• Introduction and Background

• Databases
• Species Filtering
• Intact Protein MW Filtering
• Intact Protein pI Filtering
• Accession Number Filtering
• Species Code Filtering
• Enzyme specificity / Missed cleavages
• Frame Translation in DNA databases

Introduction and Background

• the total number of entries in the database;
• the number of entries within the selected molecular weight range;
• the number of entries within the selected pI range;
• the number of entries for the selected species;
• the number of entries fitting the combined molecular weight, species and pI parameters.

• the index number of the longest protein;
• the number of amino acids in the longest protein;
• the mass of the longest protein;
• the index number of the protein with the most digest fragments;
• the number of digest fragments for the protein with the most digest fragments;
• the total number of digest fragments;
• the average protein mass.