MS-Fit Instructions

A peptide mass fingerprinting tool from the that tries to fit a user's mass spectrometry data to a protein sequence in an existing database and thus suggest the identity of the user's protein. The MS input data should be generated by analyzing the peptides produced by the enzymatic digestion of a user's protein.
MS-Seq MS-Pattern
MS-Digest MS-Product MS-Comp DB-Stat MS-Isotope
Database: DNA Frame Translation:

Accession Numbers:
Digest:
      Max. # of missed cleavages:
Cysteines modified by:
N term: C term:
Species:
MW of Protein: (from Da to Da) All
Protein pI: (from to ) All
Names:
Max. Reported Hits:
Sample ID (comment):
Sort By:
Min. # peptides required to match:
Report MOWSE Scores: Pfactor:
Possible
Modifications
Mode (default)
User Defined Modification 1:
OR
Homology Mode (select any mode but identity)
Search mode:
Min. # matches with NO AA substitutions:
Peptide Mass shift: Da

Instrument:
Peptide masses are:
Mass Tolerance:
Contaminant Masses:
Mass (m/z)             Charge (z)
Other peptide-mass fingerprinting servers:
Prediction of potential protein post-translational modifications and single amino acid substitutions: